Journal article
Analysis of Wnt signalling dynamics during colon crypt development in 3D culture
CW Tan, Y Hirokawa, AW Burgess
Scientific Reports | NATURE PORTFOLIO | Published : 2015
DOI: 10.1038/srep11036
Abstract
Many systems biology studies lack context-relevant data and as a consequence the predictive capabilities can be limited in developing targeted cancer therapeutics. Production of colon crypt in vitro is ideal for studying colon systems biology. This report presents the first production of, to our knowledge, physiologically-shaped, functional colon crypts in vitro (i.e. single crypts with cells expressing Mucin 2 and Chromogranin A). Time-lapsed monitoring of crypt formation revealed an increased frequency of single-crypt formation in the absence of noggin. Using quantitative 3D immunofluorescence of β-catenin and E-cadherin, spatial-temporal dynamics of these proteins in normal colon crypt ce..
View full abstractRelated Projects (2)
Grants
Awarded by Ludwig Institute for Cancer Research
Funding Acknowledgements
The authors wish to thank Dr Anuratha Sakthianandeswaren and Dr Michael Buchert (The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia) for kindly providing APC<SUP>min/+</SUP> and Tg(A33-CreERT2); Apc<SUP>fl/fl</SUP> mice colon respectively, during this study. We acknowledge funding from the National Health and Medical Research Council Project Grant 1029628, Program Grant 487922, funds from the Operational Infrastructure Support Program provided by the Victorian Government and ongoing support from the Ludwig Institute for Cancer Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Additionally the manuscript is solely the responsibility of the institutions and individual authors and does not reflect the views of NHMRC.